What question did this study set out to answer?

To develop and evaluate a targeted polymeric gene delivery carrier specific to angiogenic endothelial cells using an integrin-binding RGD peptide conjugated to polyethyleneimine via a PEG spacer.

October 28, 2002Molecular TherapyOpen Access

An Angiogenic, Endothelial-Cell-Targeted Polymeric Gene Carrier

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Authors

WSWonhee SuhChung-Ang University SHSeonggyun HanUniversity of Utah LYLan YuUniversity of Utah

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Implication

In vitro experimental study demonstrates enhanced gene delivery using RGD-conjugated polyethyleneimine in angiogenic endothelial cells, suggesting a targeted strategy for vascular therapies.

Key Points

To develop and evaluate a targeted polymeric gene delivery carrier specific to angiogenic endothelial cells using an integrin-binding RGD peptide conjugated to polyethyleneimine via a PEG spacer.
Conjugated an αvβ3/αvβ5 integrin-binding RGD peptide (ACDCRGDCFC) to polyethyleneimine (PEI) via a poly(ethylene glycol) (PEG) spacer to form PEI-g-1PEG-RGD.
Evaluated particle size, aggregation, and surface charge of the resulting polymer-DNA complexes at neutral charge ratios.
Assessed in vitro gene transfection efficiency and targeting specificity in angiogenic versus normal/angiostatic endothelial cells, comparing PEI-g-1PEG-RGD to unmodified PEI and a non-targeting PEI-g-1PEG-RAE control.
PEI-g-1PEG-RGD formed homogeneous DNA complexes with an effective diameter of 100–200 nm and reduced surface charge, avoiding the aggregation observed with unmodified PEI.
PEI-g-1PEG-RGD achieved an approximately fivefold increase in transfection efficiency over unmodified PEI in angiogenic endothelial cells via integrin-mediated internalization.
PEI-g-1PEG-RGD demonstrated high selectivity for angiogenic endothelial cells compared to normal endothelial cells.

Cite This Study

Suh et al. (2002) studied this question.

synapsesocial.com/papers/6a72b82b2163a0a01bc642cf https://doi.org/10.1016/s1525-0016(02)90721-5

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Implication

In vitro experimental study demonstrates enhanced gene delivery using RGD-conjugated polyethyleneimine in angiogenic endothelial cells, suggesting a targeted strategy for vascular therapies.

An Angiogenic, Endothelial-Cell-Targeted Polymeric Gene Carrier

Discussion

Member takes

Implication

Key Points

Cite This Study

An Angiogenic, Endothelial-Cell-Targeted Polymeric Gene Carrier

Discussion

Member takes

Implication

Key Points

Cite This Study

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