What question did this study set out to answer?

To elucidate the molecular mechanism by which cellular transcription factors YY1 and LSF cooperate to repress HIV-1 long terminal repeat transcription and establish viral latency.

August 1, 2000Journal of VirologyOpen Access

The Human Factors YY1 and LSF Repress the Human Immunodeficiency Virus Type 1 Long Terminal Repeat via Recruitment of Histone Deacetylase 1

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Authors

JCJason J. CoullThe University of Texas Southwestern Medical Center FRFabio RomerioUniversity of Maryland, Baltimore JSJian-Min SunUniversity of Manitoba

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Implication

Laboratory study reveals that YY1 and LSF recruit histone deacetylase 1 to silence HIV-1 promoter transcription, suggesting a molecular mechanism for establishing viral latency.

Key Points

To elucidate the molecular mechanism by which cellular transcription factors YY1 and LSF cooperate to repress HIV-1 long terminal repeat transcription and establish viral latency.
Characterized protein-protein interactions and DNA-binding activity between recombinant YY1 zinc finger domains, wild-type LSF, and DNA-binding-deficient LSF mutants at the HIV-1 long terminal repeat.
Assayed the physical interaction and copurification of histone deacetylase 1 with the YY1-LSF repressor complex, measuring promoter activity in the presence of histone deacetylase 1 overexpression and the chemical inhibitor trichostatin A.
LSF recruited YY1 to the HIV-1 long terminal repeat via the first two zinc fingers of YY1, whereas an LSF mutant unable to bind DNA completely abolished promoter repression.
Histone deacetylase 1 copurified with the YY1-LSF complex, and its overexpression amplified transcriptional repression, while treatment with trichostatin A fully blocked YY1-dependent repression.

Cite This Study

Coull et al. (2000) studied this question.

synapsesocial.com/papers/6a72b8e0febe604dd70abd28 https://doi.org/10.1128/jvi.74.15.6790-6799.2000

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Implication

Laboratory study reveals that YY1 and LSF recruit histone deacetylase 1 to silence HIV-1 promoter transcription, suggesting a molecular mechanism for establishing viral latency.

The Human Factors YY1 and LSF Repress the Human Immunodeficiency Virus Type 1 Long Terminal Repeat via Recruitment of Histone Deacetylase 1

Discussion

Member takes

Implication

Key Points

Cite This Study

The Human Factors YY1 and LSF Repress the Human Immunodeficiency Virus Type 1 Long Terminal Repeat via Recruitment of Histone Deacetylase 1

Discussion

Member takes

Implication

Key Points

Cite This Study

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