January 1, 1996Biochemistry

Tropomyosin-Binding Site(s) on the Dictyostelium Actin Surface As Identified by Site-Directed Mutagenesis

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Population

Dictyostelium cells expressing mutant actins (mutant 645, mutant 663, mutant 647, chimeric actin mutant 646)

Comparison

Site-directed mutagenesis of actin subdomain 4 vs Rabbit skeletal actin and control mutants

Design

Preclinical

Authors

KSKimiko SaekiTokyo University of Science KSKazuo SutohWaseda University TWTakeyuki WakabayashiThe University of Tokyo

Discussion

Member takes

Implication

Subdomain 4 remains an investigational target in cardiac thin-filament regulation; extends structural mapping but leaves translation to native systems open.

Structured PICO

Population

Dictyostelium cells expressing mutant actins (mutant 645, mutant 663, mutant 647, chimeric actin mutant 646)

Intervention

Site-directed mutagenesis of actin subdomain 4 (e.g., QTAAS-to-KAYKE replacement, K238A/E241A/E360H)

Comparator

Rabbit skeletal actin and control mutants (mutant 647, mutant 663)

Outcome

Tropomyosin binding and activation of myosin ATPase activitysurrogate

Site-directed mutagenesis reveals that the tropomyosin-binding sites of 'on'-state actin are located on subdomain 4.

Cite This Study

Saeki et al. (1996) studied this question.

synapsesocial.com/papers/6a72c2d2f44fa9f079e0219d https://doi.org/10.1021/bi961292c

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Implication

Subdomain 4 remains an investigational target in cardiac thin-filament regulation; extends structural mapping but leaves translation to native systems open.

Tropomyosin-Binding Site(s) on the Dictyostelium Actin Surface As Identified by Site-Directed Mutagenesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Tropomyosin-Binding Site(s) on the Dictyostelium Actin Surface As Identified by Site-Directed Mutagenesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider