N- [(4(or 7)-Methoxy-2-benzimidazolyl) methyl] maleimide (IIIa) was synthesized as"fluorescence-labeled"protein-sulfhydryl reagent. Polyphosphoric acid was proposed to be a general reagent for imide cyclization reaction. Fluorescent characteristics of some benzimidazole derivatives were described.
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Kanaoka et al. (1964) studied this question.