What question did this study set out to answer?

To determine whether free-radical oxidation of low-density lipoproteins generates carboxyalkylpyrrole epitopes and evaluate their clinical presence in human plasma.

December 1, 1997Chemical Research in Toxicology

(Carboxyalkyl)pyrroles in Human Plasma and Oxidized Low-Density Lipoproteins

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Authors

KKKamaljit KaurChandigarh University RSRobert G. SalomonCase Western Reserve University JOJune O’NeilCleveland Clinic

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Implication

Observational study reveals elevated carboxyalkylpyrroles in patients with atherosclerosis and renal failure, highlighting novel biomarkers of lipid oxidation.

Key Points

To determine whether free-radical oxidation of low-density lipoproteins generates carboxyalkylpyrrole epitopes and evaluate their clinical presence in human plasma.
Developed specific enzyme-linked immunosorbent assays using antibodies raised against keyhole limpet hemocyanin-bound CHP and CPP.
Oxidized arachidonic and linoleic acids in the presence of human serum albumin and measured epitope yield with and without KOH ester hydrolysis.
Quantified protein-bound CHP immunoreactivity in plasma samples from healthy controls and patients diagnosed with renal failure or atherosclerosis.
Oxidation of linoleic acid selectively generated albumin-bound CHP, whereas arachidonic acid oxidation generated albumin-bound CPP, both showing under 0.5% antibody cross-reactivity.
KOH-mediated ester hydrolysis markedly elevated immunoreactive epitope detection in oxidized LDL, indicating carboxyalkylpyrroles originate from oxidized core and surface lipid esters.
Protein-bound CHP immunoreactivity was significantly elevated in plasma from patients with atherosclerosis and renal failure relative to healthy volunteers.

Cite This Study

Kaur et al. (1997) studied this question.

synapsesocial.com/papers/6a72d8211087f344d91ae50c https://doi.org/10.1021/tx970112c

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Implication

Observational study reveals elevated carboxyalkylpyrroles in patients with atherosclerosis and renal failure, highlighting novel biomarkers of lipid oxidation.

(Carboxyalkyl)pyrroles in Human Plasma and Oxidized Low-Density Lipoproteins

Discussion

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Implication

Key Points

Cite This Study

(Carboxyalkyl)pyrroles in Human Plasma and Oxidized Low-Density Lipoproteins

Discussion

Member takes

Implication

Key Points

Cite This Study

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