Unphazed and Phogo: complete genome sequences of two EK1 cluster Podoviridae Microbacterium phages containing putative purple acid phosphatase genes

Brett M. Condon; Jacob D. Ariel; Saundra L. Godshall; Drithi Patel; Matthew T. McDonald; Susan M. R. Gurney; Minahil Chaudhry; Amy Chin; Elaine Ficarra; Anjani Guda; Alexandra Heck; Devon M. Moore; Manasa Mula; Jamie M. O'Donnell; Akhil Patel; Mansi Patel; Svetlana Pauley; Anish K. Ponna; Sunny H. Shastri; Riya M. Solanky

doi:10.1128/mra.00423-26

Key Points

This research aims to characterize two newly isolated EK1 cluster bacteriophages and their genomic features.
Isolated bacteriophages from soil samples using Microbacterium foliorum as the host.
Conducted electron microscopy imaging to observe podovirus morphology.
Performed genome sequencing and annotation for genetic analysis.
Both phages display podovirus morphology as observed via electron microscopy.
The genomic analysis revealed similarities with existing EK1 cluster phages.
Both phages contain a putative purple acid phosphatase (PAP) gene, which is also expressed in plants.

Implication

Genome sequencing reveals the characteristics of bacteriophages from soil samples, highlighting their potential functions.

Abstract

We report two EK1 cluster bacteriophages isolated from soil samples using the host Microbacterium foliorum . EM imaging showed that both exhibit the podovirus morphology. Genome sequencing and annotation showed similarity with many EK1 phage genomes. Our phages contain a putative PAP gene, also expressed in plants.

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Cite This Study

Condon et al. (2026) studied this question.

synapsesocial.com/papers/6a72e85b226790f37065834e https://doi.org/10.1128/mra.00423-26

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Unphazed and Phogo: complete genome sequences of two EK1 cluster Podoviridae Microbacterium phages containing putative purple acid phosphatase genes

Key Points

Implication

Abstract

Discussion

Cite This Study

Unphazed and Phogo: complete genome sequences of two EK1 cluster Podoviridae Microbacterium phages containing putative purple acid phosphatase genes

Key Points

Implication

Abstract

Discussion

Cite This Study

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