Isolation and Molecular Detection of Pathogenic Vibrio Species among Economic Fish from Red Sea in Egypt

A total of 105 samples were collected from Siganus rivulatus, Mulloidichthys vanicolensis, and Lethrinus lentjan, freshly captured from the Red Sea along Hurghada City coastline zone, Egypt. Clinical and post mortem findings revealed the presence of characteristic clinical signs and lesions similar to those reported in vibriosis. Out of 43 putative Vibrio species isolates obtained by culturing; 30 isolates were presumptively discriminated into Vibrio cholera (n=11), Vibrio anguillarum (n=8), Vibrio fluvialis/ Vibrio furnissii (n=4), Vibrio harveyi (Vibrio carchariae) (n=4) and Vibrio alginolyticus (n=3), but it was not initially possible to approve or repudiate that the remaining 13 isolates were Vibrio species through phenotypic characterization. By using PCR, targeting