Purification and Characterization of Strawberry Crinkle Virus

(...) Electron microscopic examination of these purified preparations revealed the presence of bacilliform particles and an apparent size difference between the two classes. Polyacrylamide gel electrophoresis of sodium dodecyl sulfate-disrupted virions showed that SCV contains three major structural proteins with molecular weights estimated to be 78, 47, and 25 kDa. The 78-kDa protein bound lectins, identifying it as the viral glycoprotein. The SCV protein profile resembles that of lettuce necrotic yellows virus (LNYV) but differs appreciably from sonchus yellow net virus (SYNV). SCV has no detectable serological relatedness to LNYV or SYNV. These results suggest that SCV is distinct from other previously characterized rhabdoviruses