Fecal microbiota transplantation from obese Jinhua pigs significantly increased serum triglyceride levels (1.68 vs 1.18 mmol/L) and hepatic lipogenesis in mice compared to microbiota from lean Landrace pigs.
Does fecal microbiota transplantation from obese pigs increase adiposity and lipogenesis in mice compared to microbiota from lean pigs?
Gut microbiota from obese pigs enhances lipogenesis and adiposity when transplanted into mice, demonstrating that gut microbiota is a major contributor to adiposity.
Absolute Event Rate: 1.68% vs 1.18%
p-value: p=0.050
Different breeds of pigs vary greatly in their propensity for adiposity. Gut microbiota is known to play an important role in modulating host physiology including fat metabolism. However, the relative contribution of gut microbiota to lipogenic characteristics of pigs remains elusive. In this study, we transplanted fecal microbiota of adult Jinhua and Landrace pigs, two breeds of pigs with distinct lipogenic phenotypes, to antibiotic-treated mice. Our results indicated that, four weeks after fecal transplantation, the mice receiving Jinhua pigs’ “obese” microbiota (JM) exhibited a different intestinal bacterial community structure from those receiving Landrace pigs’ “lean” microbiota (LM). Notably, an elevated ratio of Firmicutes to Bacteroidetes and a significant diminishment of Akkermansia were observed in JM mice relative to LM mice. Importantly, mouse recipients resembled their respective porcine donors in many of the lipogenic characteristics. Similar to Jinhua pig donors, JM mice had elevated lipid and triglyceride levels and the lipoprotein lipase activity in the liver. Enhanced expression of multiple key lipogenic genes and reduced angiopoietin-like 4 (ANGPTL4) mRNA expression were also observed in JM mice, relative to those LM mice. These results collectively suggested that gut microbiota of Jinhua pigs is capable of enhancing lipogenesis than that of Landrace pigs. Transferability of the lipogenic phenotype across species further indicated that gut microbiota plays a major role in contributing to adiposity in pigs. Manipulation of intestinal microbiota will, therefore, have a profound impact on altering host metabolism and adipogenesis, with an important implication in the treatment of human overweight and obesity.
Yang et al. (Mon,) conducted a other in Adiposity (n=24). Fecal microbiota transplantation from Jinhua pigs vs. Fecal microbiota transplantation from Landrace pigs was evaluated on Serum triglyceride level (mmol/L) (p=0.050). Fecal microbiota transplantation from obese Jinhua pigs significantly increased serum triglyceride levels (1.68 vs 1.18 mmol/L) and hepatic lipogenesis in mice compared to microbiota from lean Landrace pigs.