What question did this study set out to answer?

To estimate the growth fraction of proliferating cells relative to non-dividing cells in spontaneous mouse mammary tumors using an autoradiographic labeling approach.

May 1, 1962JNCI Journal of the National Cancer Institute

Autoradiographic Analysis of Cell Proliferation in Spontaneous Breast Cancer of C3H Mouse. III. The Growth Fraction²

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To estimate the growth fraction of proliferating cells relative to non-dividing cells in spontaneous mouse mammary tumors using an autoradiographic labeling approach.
Administered parenteral injections of tritiated thymidine to C3H mice bearing spontaneous mammary tumors five days prior to tumor fixation or autotransplantation.
Determined growth fraction by calculating the ratio between the total labeled cell index and the labeled mitotic index.
Modeled the mathematical impacts of variability in generation times, mitosis duration, and DNA synthesis duration on growth fraction calculations.
Tumors examined five days post-injection yielded a mean time-dependent growth fraction estimate of 0.61.
Adjusting for tumor volume doubling times of one week or autotransplantation kinetics established an initial baseline growth fraction of 0.40.
Theoretical simulations demonstrated that variations in generation time preserve the model's validity when mitosis or DNA synthesis durations vary proportionally, but cause marked underestimation when synthesis durations remain fixed.

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Implication

Preclinical autoradiography study reveals a 40 percent initial growth fraction in spontaneous mouse breast cancer, suggesting most tumor cells do not actively proliferate simultaneously.

Cite This Study

Mortimer L. Mendelsohn (1962) studied this question.

synapsesocial.com/papers/6a73353bd7374855d0c8f532 https://doi.org/10.1093/jnci/28.5.1015

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Implication

Preclinical autoradiography study reveals a 40 percent initial growth fraction in spontaneous mouse breast cancer, suggesting most tumor cells do not actively proliferate simultaneously.

Autoradiographic Analysis of Cell Proliferation in Spontaneous Breast Cancer of C3H Mouse. III. The Growth Fraction²

Key Points

Discussion

Implication

Cite This Study

Autoradiographic Analysis of Cell Proliferation in Spontaneous Breast Cancer of C3H Mouse. III. The Growth Fraction²

Key Points

Discussion

Implication

Cite This Study

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