Comparing the accuracy of ultrasound-based measurements of the cervical vagus nerve

Figures

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  Figure 1. Overview on morphological findings derived from casting (cross-sectional area, diameter, and shape) and histology (nerve fascicle count, vascularity, and morphological composition; results with standard deviation; for diameter, shape, and morphological composition the average of both body sides is given).

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  Figure 2. Histology sample of the cervical vagus nerve stained with hematoxylin–eosin. Nerve fascicles surrounded by perineurium and epineurium can be seen (A). Vascular supply with the “vagal artery” laying external to the epineurium (B), subperineural vessels within the perineurium (C), and minor vessels within the axon tissue within the nerve’s fascicles (D) are shown. Pictures of (B–D) are magnifications of (A) in given resolution.

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  Figure 3. Box plots comparing different methods to determine the cervical vagus nerve’s cross-sectional area in consideration of the epineurium. Dotted Boxes indicate the left side, striped boxes the right side. The boxes show the 25th, 50th and 75th percentile, whiskers the minima and maxima. The solid line marks the median. Black—ultrasound (US), darker grey—cast, lighter grey—histology (Histo); Epi: Measurements contain the epineurium.

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  Figure 4. Determining the cervical vagus nerve cross-sectional area with ultrasound, cast, and histology. Interindividual differences donor- and side-depended were detected. Black—ultrasound darker grey—casting, lighter grey—histology. Dotted lines indicate the left and full lines the right body side.

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  Figure 5. Bland–Altman plots showing the differences of measurement between the applied methods for cross-sectional area detection. Most values lied within the 95% confidence interval (LOA) yielding that differences are distributed within close borders. Ultrasound (US) and casting (Cast) with epineurium (Epi) showed negative bias (A), ultrasound versus histology (Histo) likewise a negative bias (B), and casting with epineurium versus histology a positive bias for both body sides (C). LOA Limits of agreement.

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  Figure 6. Five steps showing the workflow of analyzing the cervical vagus nerve’s cross-sectional area.

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  Figure 7. (A) The cervical vagus nerve (CVN), the internal jugular vein (IJV), and the common carotid artery (CCA) are shown using high-resolution ultrasound. The yellow arrow points out the nerve’s hyperechoic epineurium. The nerve’s cross-sectional area is measured within this structure (scale in mm). (B) Preparation of the common carotid artery (1), the cervical vagus nerve (2), and the internal jugular vein (3). Most often the nerve is found in between the great vessels. (C) Casting of the cervical vagus nerve with pink-colored dental molding silicon. The blue mold provides sufficient casting by separating the nerve from surrounding tissue and moisture. The black line marks the level of examination.