Permanent Re-Entrant Diversion of Porcine Pancreatic Secretions

A surgical procedure was developed to permit improved long-term collection of exocrine pancreatic secretions. A cannula was used to conduct pancreatic juice from a small isolated segment of duodenum which received the pancreatic duct, through permanent intercostal fistulas, and back into the duodenum within 3 cm of the normal entry point for pancreatic juice. Success with this procedure is attributed to fistula location, one-way valve design of the re-entrant arm of the cannula and precautions taken to minimize the formation of intra-abdominal adhesions. Pancreatic juice was collected from three barrows (35 kg initial weight) which were fed three cornstarch-based diets (high fat — 14% crude protein, 10% tallow; control — 14% crude protein, 2% tallow; protein-free — 0% crude protein, 2% tallow), twice daily, 0.9 kg each meal, 12 h apart for a 2-wk period. The daily volume of pancreatic juice secreted was 3.9, 4.0 and 3.5 L (n = 6) for the high fat, control and protein-free diets, respectively. In the same order for the diets, the daily protein secretion was 15.3, 15.0 and 12.2 g respectively. A 12-h secretion profile related to meal time showed a similar response pattern to feeding for all diets, i. e., secretion rates increased within the first 3 h, peaked during 3–7 h and decreased to basal rates during the last 5 h. Secretion rates obtained from blind 5-cm duodenal pouches did not exceed 20 mL per 24 h. During the 4-mo study, pancreatic secretions were persistent and meal induced secretion patterns were sustained. Cannulas were still functional when pigs achieved liveweights greater than 100 kg.