Identification of the 3′ ‐ and 5′ ‐ends of beet necrotic yellow vein virus RNAs

Beet necrotic yellow vein virus RNAs are characterized by a 5′ ‐end cap structure and polyadenylated 3′ ‐ends. The presence of a poly(A) sequence with major lengths ranging from 65–140 nucleotides could be demonstrated by homochromatography and sequencing methods. Beet necrotic yellow vein virus RNAs could be 5′ ‐end labelled with kinase only after enzymatic or chemical cap elimination followed by dephosphorylation. The 5′ ‐end structure is found to be of cap O type: m 7 GpppNp.