Key result
The reactivity of monoclonal antibodies specific to distinct myosin heavy chain isoforms varies significantly between species and between immunohistochemical and immunoblotting techniques.
The specificity of monoclonal antibodies against myosin heavy chain isoforms differs between species and experimental techniques, necessitating careful validation in muscle research.
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Requires species- and technique-specific validation of myosin antibodies; leaves open reliable cross-species cardiac muscle comparisons.
Smerdu et al. (2009) studied Healthy skeletal muscle (n=8). Monoclonal antibodies against MyHC isoforms was evaluated on Antibody specificity in immunohistochemistry and immunoblotting. The reactivity of monoclonal antibodies specific to distinct myosin heavy chain isoforms varies significantly between species and between immunohistochemical and immunoblotting techniques.
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