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Upon reduction of the oxygen‐stable hydrogenase of Desulfovibrio vulgaris strain Hildenborough this enzyme becomes oxygen sensitive. Only anaerobic oxidation of the reduced enzyme with 2,6‐dichlorophenol‐indophenol, in the presence of iron and EDTA, followed by addition of Tris‐EDTA (pH 9.0) converts the enzyme back into its oxygen‐stable state, without loss of activity.
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Dijk et al. (1983) studied this question.
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