Demonstration of at least two different actin-binding sites in villin, a calcium-regulated modulator of F-actin organization.

View Full Paper

Ask AI

Bookmark

Population

In vitro model of villin and F-actin

Design

Preclinical

Authors

Discussion

Supports distinct villin actin-binding domains in vitro; leaves open their roles in epithelial cytoskeletal dynamics in vivo.

Population

In vitro model of villin and F-actin

Intervention

In vitro proteolysis of villin to isolate core fragment and headpiece

Outcome

F-actin binding, nucleation, severing, and bundling activitysurrogate

Identifies the headpiece of villin as a distinct F-actin binding domain responsible for bundling activity.

Glenney et al. (1981) studied this question.

View Full Paper

Ask AI

Bookmark