What question did this study set out to answer?

To investigate the spatial and temporal expression patterns of vascular endothelial growth factor (VEGF) and its receptors (Flk-1 and Flt-1) in the mouse uterus throughout the peri-implantation period (days 1–8).

November 1, 1995Journal of Endocrinology

Differential expression of vascular endothelial growth factor and its receptor mRNAs in the mouse uterus around the time of implantation

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Authors

ICIndrani ChakrabortyDr. A.P.J. Abdul Kalam Technical University SDSukhen DasJadavpur University SDS K DeyMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

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Implication

Laboratory study demonstrates spatiotemporal regulation of VEGF and receptor mRNAs in mice, indicating critical roles in vascular permeability and angiogenesis.

Key Points

To investigate the spatial and temporal expression patterns of vascular endothelial growth factor (VEGF) and its receptors (Flk-1 and Flt-1) in the mouse uterus throughout the peri-implantation period (days 1–8).
Analyzed uterine tissue samples from pregnant mice across days 1 to 8 of gestation using Northern blot analysis to detect transcript sizes and overall expression levels.
Conducted in situ hybridization to map cell-specific spatial localization of VEGF, Flk-1, and Flt-1 mRNAs.
Performed immunohistochemistry for von Willebrand factor (vWF) to identify total endothelial cells and lectin (Dolichos biflorus agglutinin) binding to detect newly sprouting angiogenic vessels.
A primary ≈4.2 kb transcript of VEGF mRNA remained steady before increasing on day 8 in decidual tissue, with mRNA localizing sequentially to luminal epithelium on days 1–2, subepithelial stroma on day 4, surrounding blastocysts on day 5, and across decidual poles and trophoblasts on days 6–8.
Flk-1 (≈6.5–7.0 kb transcript) and Flt-1 (≈6.5 and 7.5 kb transcripts) mRNAs were absent on day 1 but accumulated in stromal cells by day 4 and within mesometrial and antimesometrial decidual beds on days 5–8.
Flk-1 mRNA expression localized to existing vWF-positive endothelial cells in the day 4 stroma and antimesometrial decidua, as well as to lectin-positive sprouting endothelial cells in the mesometrial pole on days 5–8.

Cite This Study

Chakraborty et al. (1995) studied this question.

synapsesocial.com/papers/6a736fe983fe59cdbfc1e8bb https://doi.org/10.1677/joe.0.1470339

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Implication

Laboratory study demonstrates spatiotemporal regulation of VEGF and receptor mRNAs in mice, indicating critical roles in vascular permeability and angiogenesis.

Differential expression of vascular endothelial growth factor and its receptor mRNAs in the mouse uterus around the time of implantation

Discussion

Member takes

Implication

Key Points

Cite This Study

Differential expression of vascular endothelial growth factor and its receptor mRNAs in the mouse uterus around the time of implantation

Discussion

Member takes

Implication

Key Points

Cite This Study

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