What question did this study set out to answer?

To investigate the driving forces and enzymatic mechanisms governing calcium accumulation in vacuolar membrane vesicles of Saccharomyces cerevisiae.

May 1, 1983Journal of Biological ChemistryOpen Access

Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

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To investigate the driving forces and enzymatic mechanisms governing calcium accumulation in vacuolar membrane vesicles of Saccharomyces cerevisiae.
Measured Ca2+ uptake kinetics in isolated yeast vacuolar membrane vesicles across various nucleotide and cation conditions.
Evaluated transport sensitivity to ionophores (SF6847, A23187, nigericin, valinomycin) and specific ATPase inhibitors (DCCD, oligomycin, sodium vanadate).
Monitored transmembrane proton gradient formation and dissipation through quinacrine fluorescence quenching assays.
Ca2+ uptake exhibited saturation kinetics with a Km of 0.1 mM and an optimal pH of 6.4, requiring Mg2+ and ATP.
Protonophores SF6847 and nigericin completely blocked transport, whereas valinomycin stimulated uptake 1.35-fold.
ATP-dependent delta-pH formation was partially dissipated by CaCl2, confirming transport driven by a Ca2+/H+ antiport system.

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Implication

In vitro experimental study demonstrates ATP-dependent calcium transport driven by proton motive force in yeast vacuolar vesicles, indicating a calcium-proton antiport mechanism.

Cite This Study

Ohsumi et al. (1983) studied this question.

synapsesocial.com/papers/6a7379914e58671dcf6c36af https://doi.org/10.1016/s0021-9258(20)81935-8

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Implication

In vitro experimental study demonstrates ATP-dependent calcium transport driven by proton motive force in yeast vacuolar vesicles, indicating a calcium-proton antiport mechanism.

Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

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Discussion

Implication

Cite This Study

Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.

Key Points

Discussion

Implication

Cite This Study

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