What question did this study set out to answer?

To determine whether the C. elegans gene lag-2 encodes a signaling ligand for the GLP-1 and LIN-12 receptors during intercellular interactions.

October 1, 1994Development

lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. elegans

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Authors

SHSamuel T. HendersonSMEC (Australia)DGDali GaoUniversity of Wisconsin–Madison ELEric J. LambieMRC Laboratory for Molecular Cell Biology

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Implication

Genetic analysis reveals LAG-2 as a membrane ligand for GLP-1 and LIN-12 in Caenorhabditis elegans, suggesting ligand uptake during cell-cell signaling.

Key Points

To determine whether the C. elegans gene lag-2 encodes a signaling ligand for the GLP-1 and LIN-12 receptors during intercellular interactions.
Analyzed the amino acid sequence of predicted LAG-2 membrane protein for homology to Drosophila Delta.
Mapped lag-2 promoter activity using reporter constructs and evaluated endogenous lag-2 mRNA distribution via in situ hybridization.
Tested whether a LAG-2::beta-galactosidase fusion protein could rescue lag-2 null mutants and tracked its localization across signaling distal tip cells and the receiving germ line.
lag-2 was found to encode a putative membrane protein with structural sequence similarity to Drosophila Delta, a known ligand for Notch.
Endogenous lag-2 transcripts and promoter-driven reporter expression localized to signaling distal tip cells rather than the germ line.
A functional LAG-2::beta-gal fusion protein fully rescued the lag-2 null phenotype and was detected within both the signaling distal tip cell and receiving germline cells.

Cite This Study

Henderson et al. (1994) studied this question.

synapsesocial.com/papers/6a73a3a371f2000f104c9eda https://doi.org/10.1242/dev.120.10.2913

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Implication

Genetic analysis reveals LAG-2 as a membrane ligand for GLP-1 and LIN-12 in Caenorhabditis elegans, suggesting ligand uptake during cell-cell signaling.

lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. elegans

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Implication

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Cite This Study

lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. elegans

Discussion

Member takes

Implication

Key Points

Cite This Study

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