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July 1, 1976Journal of Lipid ResearchOpen Access

An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives

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Authors

BPB. J. H. M. PoorthuisAmsterdam UMC Location University of AmsterdamPYPaul J. YazakiCity of HopeKHKarl Y. HostetlerUniversity of California, San Diego

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Implication

Methodological study demonstrates enhanced separation of phosphatidylglycerol and related phospholipids using boric acid-treated silica plates, indicating improved lipid profiling in tissues.

Key Points

  • To develop an improved two-dimensional thin-layer chromatography system for resolving phosphatidylglycerol and its derivatives from complex tissue total lipid extracts.
  • Designed a two-dimensional thin-layer chromatography system using silica gel G plates prepared with 0.4 M boric acid.
  • Quantified phospholipid class compositions in multiple rat tissues and a Morris 7777 hepatoma model.
  • Demonstrated linear recovery of added phosphatidylglycerol and successfully prevented cochromatography with N,N-dimethylphosphatidylethanolamine.
  • Achieved clear separation of phosphatidylglycerol, cardiolipin, and bis(monoacylglyceryl)phosphate from other tissue phospholipid components.

Cite This Study

Poorthuis et al. (1976) studied this question.

synapsesocial.com/papers/6a73ea2ef4f9256fee8decd7https://doi.org/10.1016/s0022-2275(20)34931-2
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Bis‐(monoacylglyceryl) phosphate and acyl phosphatidylglycerol isolated from human livers of lipidosis induced by 4,4′‐diethylaminoethoxyhexesterol1974 · 22 citations
  2. 2The formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D1967 · 261 citations