Systematization, symbolization and microbicidal activeness of 3-amino 5,6-dimethoxyl-2-methyl derivatives Via 4H–benzo[d] [1,3]–oxazine–4–one and quinazolin-4-(3H)-one

The concentration of 2-amino-methyl-4, 5-dimethoxybenzoate with acetic anhydride yielded the cyclic compound 2-methyl-4, 5-disubstituted-1, 3-benzo-oxazine-4-one which further produce a novel 2,3-disubstituted quinazolin-4 ones via the reaction with hydrazine hydrate. The compounds synthesized were unequivocally confirmed by means of Infrared, Nuclear Magnetic Resonance ( 1 H and 13 C), Gas Chromatography Mass Spectrophotometer and Elemental analysis. The synthesized compounds were screened against various strains of microorganism; Staphylococcus aureus , Bacillus species , Escherichia coli , Klebsiella pneumonia , Serratia marcescens, and candida albicans . Compounds 1 and 2 showed significant activity against Staphylococcus aureusand Serratia marcescens with MIC ranging from 6 – 12 mg/mL.