What question did this study set out to answer?

To develop a rapid, sensitive, and continuous spectrophotometric assay to measure muramidase activity across biological systems.

June 1, 1965Experimental Biology and Medicine

A Rapid and Sensitive Assay of Muramidase.

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Authors

RPR.M. ParryUniversity of Nebraska–Lincoln RCRamesh C. ChandanUniversity of Nebraska–Lincoln KSK.M. ShahaniUniversity of Nebraska–Lincoln

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Implication

Laboratory study demonstrates rapid continuous spectrophotometric assaying of muramidase in biological systems, highlighting high sensitivity down to 0.1 micrograms.

Key Points

To develop a rapid, sensitive, and continuous spectrophotometric assay to measure muramidase activity across biological systems.
Prepared a 25 mg% suspension of Micrococcus lysodeikticus substrate in 0.05 M NaCl and M/15 phosphate buffer at pH 6.2.
Measured the rate of bacterial lysis at 540 mμ using a Beckman DB spectrophotometer paired with a continuous chart recorder.
Enabled continuous recording of initial reaction velocities during bacterial cell lysis.
Achieved a dynamic detection range capable of measuring samples containing 0.1 to 10 μg of muramidase.

Cite This Study

Parry et al. (1965) studied this question.

synapsesocial.com/papers/6a73f6a50375516f85f167e6 https://doi.org/10.3181/00379727-119-30188

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Implication

Laboratory study demonstrates rapid continuous spectrophotometric assaying of muramidase in biological systems, highlighting high sensitivity down to 0.1 micrograms.

A Rapid and Sensitive Assay of Muramidase.

Discussion

Member takes

Implication

Key Points

Cite This Study

A Rapid and Sensitive Assay of Muramidase.

Discussion

Member takes

Implication

Key Points

Cite This Study

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