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Develop and evaluate a standard bench-top flow cytometric protocol using the green nucleic acid stain SYTO 13 for counting bacterioplankton across diverse lake water samples.

June 1, 1996Limnology and OceanographyOpen Access

Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13

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Authors

PGPaul A. del GiorgioUniversité du Québec à Montréal DBDavid F. BirdUniversité du Québec à Montréal YPYves T. PrairieUniversité du Québec à Montréal

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Implication

Methodological study demonstrates rapid and precise flow cytometric enumeration of lake bacterioplankton using SYTO 13, highlighting an efficient alternative to standard epifluorescence microscopy.

Key Points

Develop and evaluate a standard bench-top flow cytometric protocol using the green nucleic acid stain SYTO 13 for counting bacterioplankton across diverse lake water samples.
Stained lake plankton samples with SYTO 13 and quantified bacterial concentrations using a standard bench-top flow cytometer equipped with a 488 nm argon laser across cell densities of 10^5 to 10^6 ml^-1.
Evaluated measurement precision across replicate instrument counts and replicate water samples, comparing throughput and accuracy against standard epifluorescence microscopy using DAPI staining.
Flow cytometric abundance estimates ranged from 72% to 141% of counts obtained via epifluorescence microscopy with DAPI staining.
Measurement precision was 10-fold higher than epifluorescence, with an average coefficient of variation (CV) of 2.4% for replicate cytometric counts and 6.7% for replicate water samples.
Sample processing time decreased by 10-fold to 15-fold compared to standard epifluorescence microscopy protocols.

Cite This Study

Giorgio et al. (1996) studied this question.

synapsesocial.com/papers/6a73f6f1f35f65f536cdea5d https://doi.org/10.4319/lo.1996.41.4.0783

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Implication

Methodological study demonstrates rapid and precise flow cytometric enumeration of lake bacterioplankton using SYTO 13, highlighting an efficient alternative to standard epifluorescence microscopy.

Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13

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Implication

Key Points

Cite This Study

Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13

Discussion

Member takes

Implication

Key Points

Cite This Study

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