Differentiation of T Cells Induced by Preparations From Thymus and by Nonthymic Agents

Summary of Experiments Showing Generation of T Cells from Precursor Cells under the Influence of Thymic and Nonthymlc Agents Material tested for inducer activity Criterion of T cell induction Source Mouse thymus Mouse spleen Description* Expression of TL or Thy-1 antigen Fr. 2 (KK) Fr. 2 (KK) Medium II: in I: in vitro~ nu/nu mice§ Induced cells % 20 <10 20,40 Mouse thymus Calf thymus Calf thymus Calf thymus Calf thymus Calf spleen Calf muscle Rat thymus Chicken thymus Human thymus Fr. 3 Fr. 2 Ft. 3 5 6 Ft. 3 Fr. 3 Ft. 2 Fr. 2 Ft. 3 20-23 <10 <10 m Calf thymus¶ (AG) 40-45 (KK) 20-25 (AG) 40-45 (AG) 25-30 (AG) 18-22 (AG) 40-45 (AG) 40-45 (KK) 20-25 (KK) 25-30 (AG) 25-30 20 25 20-25 15-19 < 10, < l0 25 10 30 <10 Appearance of helper effect in vitroll cAMP DB-cAMP Poly A:U Endotoxin BSA, Medium Ft. 2 (KK) + Aminophylline Fr. 2 (KK) + Insulin Fr. 2 (KK) + Medium PFC/culture 5th exp. 24 ~10 Because of space limitations, details of materials and methods can be indicated for the most part only by key references to where full descriptions can be found. * Fr = fraction. (KK) = fraction prepared by Dr. K. Komuro according to Goldstein