The contribution of newly synthesized cholesterol to biliary cholesterol in the rat.

Measurement of Newly Synthesized Cholesterol in RutBile 2439directly from newly synthesized sterol under normal conditions, to determine whether the magnitude of this fraction is influenced by the rate of hepatic cholesterol synthesis and, finally, to establish whether biliary cholesterol is derived from a pool of sterol that is not in equilibrium with the remaining cholesterol of the hepatocyte. MATERIALS AND METHODS'LaboratoTIes, lnc.. Uilmington.MA).originally pwchased in the wight range D f 125-150 8arknerr (0300-1500 hours) and were allowed free access t o water and Tat c h w (Wayne , were housed i n a r m with alternating 12 h periods Of l i g h t (1500-0300 hourr) and Laboratory Animal Diets.Allied Mills.lnc..