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Develop and characterize stable, sediment-free anaerobic bacterial enrichment cultures capable of extensive dechlorination of the commercial PCB mixture Aroclor 1260.

April 1, 2006Applied and Environmental MicrobiologyOpen Access

Development and Characterization of Stable Sediment-Free Anaerobic Bacterial Enrichment Cultures That Dechlorinate Aroclor 1260

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DBDonna L. BedardRensselaer Polytechnic Institute JBJessica J. BaileySt James's University Hospital BRBrandon L. ReissRensselaer Polytechnic Institute

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Implication

Laboratory study reveals extensive Aroclor 1260 dechlorination by sediment-free bacterial cultures, indicating new avenues for targeted environmental bioremediation.

Key Points

Develop and characterize stable, sediment-free anaerobic bacterial enrichment cultures capable of extensive dechlorination of the commercial PCB mixture Aroclor 1260.
Inoculated minimal media with Aroclor 1260-contaminated river sediment primed with 2,6-dibromobiphenyl, performing successive 10% transfers to yield sediment-free cultures maintained on pyruvate, butyrate, or acetate plus H2 at 22–24°C.
Assayed PCB congener dechlorination of 50 to 500 µg/ml Aroclor 1260 using gas chromatography with an electron capture detector.
Extracted community DNA from acetate-plus-H2 cultures, amplified 16S rRNA genes, and evaluated bacterial diversity across 107 clones via restriction fragment length polymorphism analysis.
Eighth-transfer sediment-free triplicate cultures dechlorinated 76% of hexa- through nonachlorobiphenyls in 250 µg/ml Aroclor 1260 to tri- through pentachlorobiphenyls within 110 days.
Dechlorination via Process N successfully transformed at least 64 double-ring chlorinated PCB congeners, including 47 congeners containing six or more chlorine atoms.
Community profiling revealed the presence of Dehalococcoides (Chloroflexi), Thauera-like Betaproteobacteria, Geobacter-like Deltaproteobacteria, Pseudomonas species, Clostridiales, and Bacteroidetes.

Cite This Study

Bedard et al. (2006) studied this question.

synapsesocial.com/papers/6a741ed684b31c8d6d33f77b https://doi.org/10.1128/aem.72.4.2460-2470.2006

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Implication

Laboratory study reveals extensive Aroclor 1260 dechlorination by sediment-free bacterial cultures, indicating new avenues for targeted environmental bioremediation.

Development and Characterization of Stable Sediment-Free Anaerobic Bacterial Enrichment Cultures That Dechlorinate Aroclor 1260

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Cite This Study

Development and Characterization of Stable Sediment-Free Anaerobic Bacterial Enrichment Cultures That Dechlorinate Aroclor 1260

Discussion

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Implication

Key Points

Cite This Study

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