What question did this study set out to answer?

Evaluate an immunoglobulin M (IgM) capture ELISA using recombinant Bor56 protein for the serodiagnosis of acute Orientia tsutsugamushi infection.

May 1, 2003Clinical and Vaccine ImmunologyOpen Access

Evaluation of an Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Orientia tsutsugamushi Infection

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Why the study?

Does an IgM capture ELISA using recombinant Bor56 antigen accurately diagnose early Orientia tsutsugamushi infection compared to IFA in patients with acute febrile diseases?

Population

176 serum samples from patients with diagnosed cases of rickettsial disease and patients with other acute…

Comparison

Immunoglobulin M capture enzyme-linked… vs Immunofluorescence antibody assay (IFA)

Design

Cross-sectional

Authors

WJWon‐Jong JangKonkuk University MHMyung-Suk Huh KPKyung Hee ParkHannam University

Discussion

Member takes

Implication

Supports evaluation of recombinant Bor56 IgM ELISA for early scrub typhus; leaves open clinical adoption pending prospective validation.

Key Points

Evaluate an immunoglobulin M (IgM) capture ELISA using recombinant Bor56 protein for the serodiagnosis of acute Orientia tsutsugamushi infection.
Engineered an IgM capture ELISA utilizing biotinylated recombinant 56-kDa major outer membrane protein (Bor56) of O. tsutsugamushi Boryong expressed as a maltose-binding fusion protein in Escherichia coli.
Compared diagnostic accuracy against the immunofluorescence antibody assay (IFA) using 176 serum samples from patients with diagnosed rickettsial disease and other acute febrile illnesses.
The IgM capture ELISA achieved a sensitivity of 100% (31/31) in IgM IFA-positive samples and 96.3% (78/81) in IgG IFA-positive samples.
The assay demonstrated a specificity of 99%, with only 1 of 95 IFA-negative control samples yielding a positive result.

Structured PICO

Does an IgM capture ELISA using recombinant Bor56 antigen accurately diagnose early Orientia tsutsugamushi infection compared to IFA in patients with acute febrile diseases?

Population

176 serum samples from patients with diagnosed cases of rickettsial disease and patients with other acute febrile diseases

Intervention

Immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) using biotinylated recombinant 56-kDa protein of Orientia tsutsugamushi Boryong (Bor56)

Comparator

Immunofluorescence antibody assay (IFA)

Outcome

Sensitivity and specificity of the IgM capture ELISA compared to IFAsurrogate

The developed IgM capture ELISA using recombinant Bor56 antigen is a highly sensitive and specific method for the early detection of scrub typhus.

Cite This Study

Jang et al. (2003) studied this question.

synapsesocial.com/papers/6a7424c8ca963d7f80391cc8 https://doi.org/10.1128/cdli.10.3.394-398.2003

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Evaluation of an Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Orientia tsutsugamushi Infection

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Evaluation of an Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Orientia tsutsugamushi Infection

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider