The anti‐inflammatory effects of manniflavanone extracted from Garcinia buchananii bark in obesity‐induced mice model (259.3)

The stem bark of Garcinia buchananii trees is used traditionally to treat diarrhea, dysentery, weight control, and diabetes in Africa. We investigated the anti‐inflammatory and antioxidant activity of the stem bark extracts and identified manniflavanone (Mf) as the most active antioxidant and anti‐inflammatory phenolic compound among the isolated bark phenolics. Seven‐week‐old C57Bl/6 J mice (4 groups) were fed a standard chow (SC) diet and high fat (HF) diet with Mf (SCMf, HFMf) extract (0.164 mg/day) for 3 weeks. Mice were terminated after 8 weeks, fecal and colon tissue was collected for analysis of inflammatory markers and microbiota. Results showed that body mass index (BMI) of mice on SCMf was lower (3.3 Kg/m2) than mice on SC (3.75 Kg/m2). Colon IL‐8, NF‐ĸB and TNF‐α mRNA levels were downregulated in SCMf to 0.69‐, 0.68‐ and 0.49‐fold of SC control, respectively, while IL‐8 and NF‐ĸB were downregulated in the HFMf to 0.27‐ and 0.53‐fold of HF control, respectively. These results indicate that the underlying anti‐inflammatory effects of Mf are related to inhibition of NF‐ĸB and NF‐ĸB‐target genes and we are currently investigating the link with changes in fecal microbiota. Grant Funding Source : wheat project