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Examines the preparation of low molecular weight substrates, suggesting potential applications in enzymatic digestion.
Preparation of short chain polymers of N-acetylglucosamine by acid hydrolysis of chitin was undertaken. Separation of the various chain lengths of the hydrolysis breakdown products was done by column chromatography on charcoal-celite with an ethanol solvent. Preparation of the dimer and tetramer of N-acetylglucosaineN- acetylmuramic acid was undertaken by lysozyme digestion of the purified cell walls of Micrococcus lysodeikticus. Separation was again accomplished by chromatography.
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Larry Robert Brown (2026) studied this question.
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