What question did this study set out to answer?

To investigate the epigenetic mechanisms by which sirtuin 7 regulates CDKN2A/p16 gene transcription during cellular senescence.

September 27, 2022Molecular Medicine ReportsOpen Access

Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence

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Authors

SRSergio RodríguezUniversidad de Salamanca GBGisella BermúdezPontificia Universidad Javeriana DGDaniel GonzálezPontificia Universidad Javeriana

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Implication

In vitro study reveals SIRT7 represses CDKN2A/p16 via H3K18 deacetylation in human fibroblasts, suggesting an epigenetic mechanism controlling cellular senescence.

Key Points

To investigate the epigenetic mechanisms by which sirtuin 7 regulates CDKN2A/p16 gene transcription during cellular senescence.
Analyzed senescent and non-senescent human MRC5 fibroblasts using quantitative PCR, Western blotting, and chromatin immunoprecipitation (ChIP) assays.
Knocked down SIRT7 expression in MRC5 cells using small interfering RNA (siRNA) to assess the impact of deacetylase loss on histone marks and promoter activation.
Senescent MRC5 cells demonstrated marked enrichment of H3K9Ac and H3K18Ac marks at the CDKN2A/p16 locus, coinciding with significant gene overexpression relative to non-senescent cells.
SIRT7 knockdown in MRC5 cells preserved H3K18Ac enrichment and directly activated the CDKN2A/p16 promoter, confirming SIRT7 acts as an epigenetic repressor of senescence.

Cite This Study

Rodríguez et al. (2022) studied this question.

synapsesocial.com/papers/6a744de24507312cc5a7fbfa https://doi.org/10.3892/mmr.2022.12861

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Implication

In vitro study reveals SIRT7 represses CDKN2A/p16 via H3K18 deacetylation in human fibroblasts, suggesting an epigenetic mechanism controlling cellular senescence.

Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence

Discussion

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Implication

Key Points

Cite This Study

Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence

Discussion

Member takes

Implication

Key Points

Cite This Study

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