Ion-exchange high performance liquid chromatography (HPLC) with an Aminex column and photodiode-array detection was used to purify, from soybean root nodules, highly polar phenolic acid conjugates that are recalcitrant to isolation by conventional techniques based on reversed-phase chemistry. HPLC, acid and enzymatic hydrolysis, ultraviolet spectroscopy, high resolution fast atom bombardment mass spectrometry (MS) and single and tandem electrospray MS were used to identify the 4-O-β-glucosides of p-hydroxybenzoic, protocatechuic and vanillic acids (which are reported in legumes for the first time) and the 5-O-β-glucoside of gentisic acid. The four phenolic acids, as well as gallic acid and its methyl ester, were also found in soybean nodules.
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Morán et al. (1998) studied this question.