Key result
Both LRRC8A and ANO1 contribute to swelling-activated chloride currents in HEK293 cells, requiring localized intracellular Ca2+ signals mediated by GPCR-independent PLC isoforms for activation.
Why the study?
The mechanisms of volume-regulated anion channel activation remain largely unknown, and whether channels such as ANO1 and intracellular Ca2+ contribute to swelling-activated chloride current activation is unclear.
No takes yet. Share an insight, caveat, or question.
Should not yet influence clinical practice; extends cellular mechanisms but leaves open cardiovascular translation.
Liu et al. (2019) studied this question. CRISPR/Cas9 gene editing and selective pharmacology was evaluated on Activation of swelling-activated chloride current (ICl,swell). Both LRRC8A and ANO1 contribute to swelling-activated chloride currents in HEK293 cells, requiring localized intracellular Ca2+ signals mediated by GPCR-independent PLC isoforms for activation.
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