Microstructural evolution of whipped cream in whipping process observed by confocal laser scanning microscopy

It is useful to carefully observe the evolution of foam structures to elucidate the factors affecting cream during whipping. In this study, confocal laser scanning microscopy and a double dyeing technology were used to investigate the microstructural evolution of a rigid foam structure in whipped cream. The location of fat and proteins were determined according to the signals they produce at different characteristic wavelengths. Protein membranes on the surface of air bubbles were clearly observed. A simple yet comprehensive characterization of the whipping process was established according to the micrographs and supported by relevant theories. The formation of a rigid foam structure depends on foaming of the protein in the plasma phase and partial coalescence of fat globules. The formation of protein foam in the cream, creation of net structure, and system breakage and collapse phenomena occurring throughout the whole whipping evolution process was depicted and distinguished visually by different colors.