Laboratory study demonstrates nitrate-driven nitrous oxide emission in soybean root nodules, confirming Bradyrhizobium bacteroids actively denitrify external nitrate.
In the presence of nitrate, N₂O emission increased markedly from soybean roots inoculated with nosZ mutant of Bradyrhizobium japonicum, but not from soybean roots inoculated with a napA nosZ double mutant, indicating that B. japonicum bacteroids in soybean nodules are able to convert the exogenously supplied nitrate into N₂O via a denitrification pathway.
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Hirayama et al. (2011) studied this question.
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