Lytic Activities in Renal Protein Absorption Droplets
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Discussion
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Implication
Histochemical study demonstrates colocalization of lysosomal enzymes and absorbed exogenous proteins in rodent renal cells, indicating rapid intracellular degradation via digestive vacuoles.
Key Points
- Investigate the digestive cycle and localization of hydrolytic enzymes following hemoglobin and ferritin uptake in rodent renal cells.
- Administered hemoglobin intraperitoneally to mice (sampled from 1 to 48 hours) and ferritin intravenously to normal and nephrotic rats (sampled at 30 minutes, 2 hours, and 48 hours).
- Prepared frozen renal sections and processed them for acid phosphatase and thiolacetate-esterase cytochemistry followed by electron microscopy.
- Lysosomal enzymes colocalized with absorbed hemoglobin in membrane-bounded apical vacuoles within 1 hour post-injection, persisting in mature absorption droplets.
- At 48 hours post-injection, proximal tubule cells displayed an increased number of enzyme-active polymorphic residual bodies representing digestive remnants.
- In nephrotic rats, ferritin segregated within acid phosphatase- and esterase-positive lysosomes primarily in glomerular mesangial cells.
Cite This Study
Miller et al. (1964) studied this question.