What question did this study set out to answer?

To identify unique surface antigens and develop an antibody-based assay to detect atypical MRSA strains that evade identification in standard slide agglutination tests.

January 1, 1994Journal of Clinical MicrobiologyOpen Access

Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests

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Authors

PKPentti KuuselaUniversity of Helsinki PHPekka HildénUniversity of Helsinki KSKatri SavolainenUniversity of Jyväskylä

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Implication

Diagnostic study demonstrates that antibodies against a 230-kDa surface protein identify slide agglutination-negative MRSA, suggesting a novel target to improve rapid bacterial detection.

Key Points

To identify unique surface antigens and develop an antibody-based assay to detect atypical MRSA strains that evade identification in standard slide agglutination tests.
Classified 79 MRSA clinical isolates from 1980 to 1990 as agglutination-negative (n=14) or agglutination-positive (n=65) using standard slide agglutination assays detecting clumping factor and protein A.
Analyzed lysostaphin digests by SDS-PAGE and immunized rabbits with an agglutination-negative MRSA strain to generate polyclonal antiserum and purified antibodies.
Assessed antiserum agglutination specificity across the 79 MRSA isolates and 68 coagulase-negative staphylococcal control strains.
Antiserum raised against the agglutination-negative strain successfully agglutinated 100% (14/14) of agglutination-negative MRSA strains and 98.5% (64/65) of agglutination-positive MRSA strains.
Cross-reactivity with coagulase-negative staphylococci was minimal, with agglutination observed in only 1.5% (1/68) of isolates.
The antiserum and purified antibodies primarily recognized a 230-kDa surface protein and its 175-kDa proteolytic fragment, which directly agglutinated all 14 agglutination-negative MRSA strains.

Cite This Study

Kuusela et al. (1994) studied this question.

synapsesocial.com/papers/6a74908ff1e99ff85ab1c560 https://doi.org/10.1128/jcm.32.1.143-147.1994

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Implication

Diagnostic study demonstrates that antibodies against a 230-kDa surface protein identify slide agglutination-negative MRSA, suggesting a novel target to improve rapid bacterial detection.

Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests

Discussion

Member takes

Implication

Key Points

Cite This Study

Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests

Discussion

Member takes

Implication

Key Points

Cite This Study

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