Hypolipidemic and anti-inflammatory effect of chitosan in experimental induced non -alcoholic fatty liver disease in rats

The biochemical effect of Chitosan on hepatic lipid metabolism in experimental induced Nonalcoholic fattyliver disease (NAFLD) in rats was investigated. Thirty male albino rats were divided into three groups (10rats each). Group I (control group): rats was fed on normal diet. Group II (NAFLD Group): rats fed onnormal diet enriched with 1% cholesterol and 2% coconut oil. Group III (Chitosan treated group): rats fedon normal diet enriched with 1% cholesterol and 2% coconut oil and supplemented with Chitosan 5%. Bloodsamples were collected after 2, 4 and 6 weeks from the onset of chitosan treatment. All serum were directlyused for determination of total cholesterol (TC), triacylglycerol (TAG), low density lipoproteincholesterol(LDL-C), very low density lipoprotein cholesterol (VLDL-C), high density lipoproteincholesterol (HDL-C), total lipids,(TL) Phospholipids, Non esterified fatty acids (NEFA) , apo-lipoprotein-B (Apo-B), tumor necrosis factor alpha (TNF-α) , interleukin-6(IL-6 ) . the obtained results showed asignificant increased in serum TC, TAG, LDL-C, VLDL-C,TL, phospholipids, NEFA and Apo-Bconcentrations with a marked decrease in HDL-C level .Also, serum TNF-α ,IL-6 was significantlyincrease in NAFLD group was observed . The results mentioned that treatment with Chitosan to NAFLDinduced rats showed a significant change and ameliorate in all parameters nearly normal level that indicatedthe Hypolypidemic and anti-inflammatory effect of Chitosan in NAFLD induced rats.