Study on the screening, characterization and production of lipase producing bacteria from different oil mill wastes

Unique strains of lipase producing bacteria were isolated from different oil mill wastes and characterized for its morphological characters. The best lipase producing isolate was selected among 20 isolates by screening them in the selection medium. The 16S rRNA sequencing of the best isolate revealed it as Enterobacter sp. The extracellular lipase activity (3.018 U mL-1) was higher than its intracellular activity (2.207 U mL-1) when grown in the production medium. The extracellular lipase was partially purified by ammonium sulphate precipitation and dialysis and checked for its optimum activity. The enzyme showed maximum activity at alkaline pH (10.0) and at a temperature of 30°C. This alkaliphilic extracellular enzyme has huge industrial potential