April 15, 1997Proceedings of the National Academy of SciencesOpen Access

Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts

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Population

Mouse NIH 3T3 cells infected with polyoma strain 59RA (multiplicity of infection 5)

Comparison

Expression of nuclear antisense RNA from the… vs Control cells treated with aphidicolin to block…

Design

Preclinical

Authors

MKMadhur KumarCalifornia Department of Public Health GCGordon CarmichaelUConn Health

Discussion

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Implication

Hypothesis-generating for antisense RNA gene regulation; leaves open human translation from animal data alone.

Structured PICO

Population

Mouse NIH 3T3 cells infected with polyoma strain 59RA (multiplicity of infection 5)

Intervention

Expression of nuclear antisense RNA from the late strand of polyoma virus

Comparator

Control cells treated with aphidicolin to block DNA replication and prevent accumulation of late-strand antisense RNA

Outcome

Adenosine modifications (A-to-I) in early-strand transcripts and their nuclear retentionsurrogate

Nuclear antisense RNA induces extensive adenosine-to-inosine modifications in target transcripts, leading to their nuclear retention and down-regulation of gene expression.

Cite This Study

Kumar et al. (1997) studied this question.

synapsesocial.com/papers/6a74dfc2d93df8ecca0926c7 https://doi.org/10.1073/pnas.94.8.3542

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Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider