Temporal variations shape the gut microbiome ecology of the moth Brithys crini

Supplementary Fig. 1. Scheme of the main pathways related to alkaloid detoxification and microbial survival in toxic environments. Enzymes present in the samples are coloured in blue. (A) Caffeine metabolism, (B) ABC transporters, (C) Two-component system, (D) Drug metabolism – Cytochrome P450, (E) Indole alkaloid biosynthesis, (F) Quorum sensing, (G) Tropane, piperidine and pyridine alkaloid biosynthesis. Supplementary Fig. 2. Comparison of hindgut and midgut diversity and composition of B. crini. (A) Alpha-diversity (Observed features and Shannon Index). (B) Beta-diversity analysis based on Non-Metric Multidimensional Scaling (NMDS) coupled with the Bray–Curtis dissimilarity matrix. Supplementary Fig. 3. Proteobacteria and Firmicutes variations over time. (A) Boxplots of the relative abundance of Proteobacteria and Firmicutes phyla per date showing an opposite trend (B). Permuspliner plots exhibiting that Proteobacteria (blue line) and Firmicutes phyla (red line) relative abundance differed over time. The black lines represent all the permuted splines based on 99 random permutations. Supplementary Table 1. Metadata of the samples including sample ID, specific tissue, season, year, and collection date. Supplementary Table 2. Relative abundance of the most abundant taxa responsible for the functional pathways studied. Taxa are shown with their phylum, followed by the lower taxonomic level reached. Supplementary Table 3. Alpha-diversity comparison (Observed features and Shannon Index) of B. crini by year, season, season x year, collection date, and gut region. The comparison was based on the Wilcoxon signed-rank test. Supplementary Table 4. Taxa comparison (phylum and genus level) of B. crini by year, season, season x year, and collection date. The comparison was based on the ANCOM test. Supplementary Table 5. ‘Permuspliner’ (Permutation test used to check significant variation of variables over time) and ‘Trendyspliner’ (Test for a significant non-zero trend over time) analyses at genus, phylum, and KEGG pathway levels (splinectomeR package functions). Supplementary Table 6. Wilcoxon signed-rank test of the most relevant metabolic functions between seasons and season x year. Supplementary Table 7. Spearman correlation between climatic variables and alpha-diversity indexes. Ordinary Least Squares (OLS) linear regressions data between alpha-diversity indexes and climatic variables. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.