A potential prognostic prediction model of colon adenocarcinoma with recurrence based on prognostic lncRNA signatures

Figures

• 

  Fig. 1. Workflow of this study

• 

  Fig. 2. Identification of differentially expressed RNAs (DERs). a Volcano map. Blue dots indicate DERs, the red horizontal dotted line represents false discovery rate (FDR) < 0.05, the two red vertical dotted lines represent the |log fold change (FC)| > 0.263. b A bidirectional hierarchical clustering map based on DERs. White and black sample bars represent recurrence and non-recurrence colon adenocarcinoma samples, respectively

• 

  Fig. 3. Screening of signature lncRNAs and construction of recurrence colon adenocarcinoma (COAD) discriminating classifier. a Accuracy curve of the optimized signature lncRNAs using recursive feature elimination (RFE) algorithm. b Receiver operating characteristic (ROC) curve based on the support vector machine (SVM) classifier in the training dataset (left) and validation dataset (right)

• 

  Fig. 4. A lncRNA-based risk score model in the training and validation dataset. a The training dataset. b The validation dataset. The top row in each pane shows the distribution of risk score distribution. The middle row in each pane shows the survival status of colon adenocarcinoma patients. The bottom row in each pane shows the heatmap of the expression of the 6 key lncRNAs. The color, from green to red, shows low to high expression

• 

  Fig. 5. Performance evaluation of the risk score (RS) prognostic prediction model. a K-M survival analysis based on RS prognostic prediction model in the training dataset. b K-M survival analysis based on RS prognostic prediction model in the validation dataset

• 

  Fig. 6. Construction of co-expression network and functional analysis. a The co-expression network of differentially expressed (DE) lncRNAs and DEmRNAs. The red color shows the upregulated lncRNAs or mRNAs, and the green color denotes the downregulated lncRNAs or mRNAs. Squares and circles, respectively, represent lncRNAs and mRNAs. b GO and KEGG pathways of DEmRNAs. Horizontal axis and vertical axis represent the gene number and term, respectively; the color and size of the bots indicate the significant P value, and the closer the color is to red, the higher the significance