Key result
Eight lncRNAs were identified as optimal diagnostic biomarkers for colon adenocarcinoma, with XXbac-B476C20.9 demonstrating potential as an independent biomarker for both diagnosis and prognosis.
Observational
The lncRNA XXbac-B476C20.9 was identified as a potential independent biomarker for the diagnosis and prognosis of colon adenocarcinoma.
LncRNA signatures may refine COAD prognosis; leaves open prospective validation before clinical adoption.
BACKGROUND: In the study, we aimed to find key long noncoding RNAs (lncRNAs) significantly associated with the diagnosis of colon adenocarcinoma (COAD) and lncRNA signatures to provide survival risk prognosis for patients with COAD. METHODS: The lncRNA and messenger RNA (mRNA) expression profiles and clinical information of patients with COAD were obtained from the Cancer Genome Atlas database. The differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) between COAD and normal tissues were identified. The optimal diagnostic and prognostic lncRNA biomarkers for COAD were identified by using feature selection procedure and classification model. Functional enrichment analysis revealed the possible roles of mRNAs coexpressed with lncRNAs in some cancer-related biological processes and pathways. Receiver operating characteristic curve of these lncRNA biomarkers were obtained by using 10-fold cross-validation. Univariate and multivariate Cox regression analysis for these DElncRNAs were performed to obtain the lncRNAs related to overall survival time. The expression levels of selected DElncRNAs were validated by quantitative real time polymerase chain reaction (qRT-PCR). RESULTS: A total of 169 DElncRNAs (60 downregulated and 109 upregulated) and 1236 DEmRNAs (708 downregulated and 528 upregulated) between COAD and normal tissues were identified. Eight lncRNAs of XXbac-B476C20.9, PP7080, CDKN2B-AS1, LINC00092, CA3-AS1, HAND2-AS1, CTD-2269F5.1, and LINC01082 were selected as optimal diagnostic lncRNA biomarkers for COAD. The expression of eight optimal diagnostic lncRNA biomarkers in qRT-PCR validation was consistent with our integrated analysis. Among them, XXbac-B476C20.9 was not only one of the eight optimal diagnostic lncRNA biomarkers, but also related to the prognosis of COAD. CONCLUSION: Our study demonstrated the promising potential of XXbac-B476C20.9 as an independent biomarker for diagnosis and prognosis of patients with COAD.
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Huang et al. (2018) conducted an observational in Colon adenocarcinoma (COAD). lncRNA biomarkers (including XXbac-B476C20.9) vs. Normal tissues was evaluated on Diagnosis and overall survival time (prognosis). Eight lncRNAs were identified as optimal diagnostic biomarkers for colon adenocarcinoma, with XXbac-B476C20.9 demonstrating potential as an independent biomarker for both diagnosis and prognosis.
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