Screening key lncRNAs and mRNAs for left-sided and right-sided colon adenocarcinoma based on lncRNA-mRNA functional synergistic network

Figures

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  Figure 1. Hierarchical clustering analysis of top 100 DElncRNAs and DEmRNAs between RSCOAD and LSCOAD. (A) DElncRNAs; (B) DEmRNAs. Row and column represented DElncRNAs/DEmRNAs and tissue samples, respectively. Orange and light blue color mean the RSCOAD and LSCOAD, respectively. The color scale represented the expression levels. Red color represents the relative expression level of genes was higher than mean, and green color represents the relative expression of genes was lower than mean. DElncRNAs, differentially expressed lncRNAs; DEmRNAs, differentially expressed mRNAs; RSCOAD, right-sided colon adenocarcinoma; LSCOAD, left-sided colon adenocarcinoma.

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  Figure 2. Identification of optimal lncRNA biomarkers between RSCOAD and LSCOAD. (A) Hierarchical clustering analysis of 31 DElncRNAs. Row and column represented DElncRNAs and tissue samples, respectively. The color scale represented the expression levels; (B) the ROC results of these 31 diagnostic lncRNA biomarker based on random forest model. DElncRNAs, differentially expressed lncRNAs; RSCOAD, right-sided colon adenocarcinoma; LSCOAD, left-sided colon adenocarcinoma.

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  Figure 3. The co-expressed DElncRNAs-DEmRNAs network. The ellipses and rhombuses were represented the DEmRNAs and DElncRNAs, respectively. Red and blue color represented up- and down-regulation, respectively. The blue and black border indicates top10 Up and Down, respectively. DElncRNAs, differentially expressed lncRNAs; DEmRNAs, differentially expressed mRNAs.

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  Figure 4. The enrichment GO terms and KEGG pathways of DEmRNAs between RSCOAD and LSCOAD. The X-axis shows –log FDR and Y-axis shows GO terms and KEGG pathways. (A) GO terms; (B) KEGG pathways. DEmRNAs, differentially expressed mRNAs; RSCOAD, right-sided colon adenocarcinoma; LSCOAD, left-sided colon adenocarcinoma; FDR, false discovery rate.

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  Figure 5. DElncRNAs-nearby DEmRNAs interaction network. The ellipses and inverted triangles were represented the DEmRNAs and DElncRNAs, respectively. Red and blue color represented up- and down-regulation, respectively. The blue and black border indicates top 10 up and down, respectively. DElncRNAs, differentially expressed lncRNAs; DEmRNAs, differentially expressed mRNAs.

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  Figure 6. Interaction network showing the overlap of DElncRNAs-DEmRNAs co-expression network with DElncRNAs-nearby DEmRNAs interaction network. The ellipses and inverted triangles were represented the DEmRNAs and DElncRNAs, respectively. Red and blue color represented up- and down-regulation, respectively. The blue and black border indicates top 10 up and down, respectively. DElncRNAs, differentially expressed lncRNAs; DEmRNAs, differentially expressed mRNAs.

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  Figure 7. Validation DEmRNAs and DElncRNAs by qRT-PCR. All of the assays were performed three times independently at least. The X-axis shows DEmRNAs or DElncRNAs and Y-axis shows log2 (fold change). The log2 (fold change) >0 and log2 (fold change) <0 indicates up-regulation and down-regulation, respectively. DElncRNAs, differentially expressed lncRNAs; DEmRNAs, differentially expressed mRNAs.

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  Figure 8. Survival curves by univariate Cox and multivariate Cox regression analysis. (A) Age; (B) neoplasm cancer status; (C) CEA level; (D) residual tumor; (E) M; (F) LSCOAD and RSCOAD. CEA, carcinoembryonic antigen; RSCOAD, right-sided colon adenocarcinoma; LSCOAD, left-sided colon adenocarcinoma.