Key result
Deoxyglucose 6-phosphate inhibits ~50% of cardiac phosphorylase activity after 5 minutes of ischemia.
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The uptake and subsequent phosphorylation of deoxyglucose into perfused rat hearts was monitored by 31P n.m.r. 2. The accumulated deoxyglucose 6-phosphate provided (a) an independent method for measuring cytosolic pH in the normoxic and ischaemic heart tissue and (b) a way of studying the activity of phosphorylase during ischaemia. 3. The cytosolic pH measured from the 31P n.m.r. resonance position of deoxyglucose 6-phosphate is in good agreement under all conditions studied with that obtained previously from the Pi resonances. This eliminates any possible doubts about the use of Pi for measuring intracellular pH. 4. Deoxyglucose 6-phosphate in vitro inhibits phosphorylase b but not phosphorylase a. Its inhibitory effect on glycogenolysis during ischaemia is monitored by measuring tissue acidosis by n.m.r. In the initial stages of ischaemia phosphorylase activity is not inhibited, whereas after about 5 min approx. 50% of the activity is inhibited. These observations are interpreted in terms of the relative contributions of phosphorylase a and the AMP-dependent phosphorylase b activities during ischaemia.
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Bailey et al. (1981) studied Total global myocardial ischaemia. Deoxyglucose (accumulated as deoxyglucose 6-phosphate) vs. Inorganic phosphate (Pi) resonances / normoxic and uninhibited states was evaluated on Inhibition of phosphorylase activity and cytosolic pH during ischaemia. Deoxyglucose 6-phosphate did not inhibit rat heart phosphorylase initially during global ischaemia, but inhibited approximately 50% of activity after about 5 minutes.
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