Myosin possesses recognition site(s) necessary for the regulation of the ATPase activity of actomyosin, demonstrating its role in calcium sensitization.
Answers are grounded in this paper and cite their sources.
Myosin modification affects Ca2+ sensitivity in vitro; leaves open relevance to intact cardiac muscle or clinical translation.
Dinitrophenylated myosin which had incorporated 1.5 moles of 1-fluoro-2,4-dinitrobenzene per 5 x 10" g of protein lost its Ca2f sensitivity when tested in an ATPase assay system containing actin, tropomyosin, troponin, and ethylene glycol his@-aminoethyl ether)-N, iV'-tetraacetic acid.Partial thiolysis of the 2,4-dinitrophenyl groups from the myosin with 2-mercaptoethanol restores its Ca2+ sensitivity.These results indicate that myosin possesses recognition site(s) necessary for the regulation of the ATPase activity of actomyosin.
No takes yet. Share an insight, caveat, or question.
Bailin et al. (1973) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: