Involvement of the 50-kDa peptide of myosin heads in the ATPase activity revealed by fluorescent modification with 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole.

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Population

Myosin subfragment-1 (S-1) ATPase

Comparison

Fluorescent modification with… vs Unmodified S-1 (original activity)

Design

Preclinical

Authors

Discussion

Hypothesis-generating for myosin domain function; leaves open translation to human cardiac therapies.

Population

Myosin subfragment-1 (S-1) ATPase

Intervention

Fluorescent modification with 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F)

Comparator

Unmodified S-1 (original activity)

Outcome

ATPase activity (K+-, Ca2+-, Mg2+-, and actin-activated Mg2+-ATPase)surrogate

Fluorescent modification reveals that the 50-kDa peptide of myosin subfragment-1 is directly involved in the ATPase reaction.

Toshiaki Hiratsuka (1986) studied this question.

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