Population
Myosin subfragment 1 (S-1) fluorescently labeled at its rapidly reacting thiol ("SH1")
Comparison
Tryptic digestion vs Uncut labeled S-1 (normal S-1)
Design
Preclinical
Authors
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No immediate clinical implications from this animal study; leaves open effects of S-1 cleavage on cardiac contractility in vivo.
Tryptic digestion of myosin subfragment-1 reduces its actin affinity for the S-1.ADP.P species by 13.4-fold compared to uncut S-1, while maintaining similar F-actin binding and excited-state lifetime.
Botts et al. (1982) studied this question.
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