A new, smaller actin-activatable myosin subfragment 1 which lacks the 20-kDa, SH1 and SH2 peptide.

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Population

Myosin filaments (in vitro biochemical model)

Comparison

Trypsin treatment at 4 degrees C in the presence… vs Standard S1 fraction

Design

Preclinical

Authors

Discussion

Challenges assumptions on SH1/SH2 thiols and 20-kDa region in myosin ATPase; leaves open translation to cardiac muscle.

Population

Myosin filaments (in vitro biochemical model)

Intervention

Trypsin treatment at 4 degrees C in the presence of 2 mM MgCl2 followed by incubation at 37 degrees C with MgATP to release S1'(75K) fraction

Comparator

Standard S1 fraction

Outcome

Actin-activated Mg2+-ATPase activity, Ca2+-ATPase, and K+-EDTA ATPase activitiessurrogate

SH1 and SH2 thiols and binding of actin to the 20-kDa region are not essential for the enhancement of Mg2+-ATPase activity in myosin.

Okamoto et al. (1987) studied this question.

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