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September 1, 1968Biochemistry

Subfragment 1 of myosin: adenosinetriphosphatase activation by actin

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Authors

EEEvan EisenbergNational Institutes of HealthCZCarsten ZobelKarlsruhe Institute of TechnologyCMCarl MoosHeinrich Heine University Düsseldorf

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Overview

Does not yet inform clinical management of cardiac contractile disorders; extends basic research on the minimal contractile unit.

Key Points

  • To examine the catalytic interaction between actin and isolated myosin subfragment 1, specifically assessing actin-mediated activation of adenosinetriphosphatase activity.
  • Purified myosin subfragment 1 and actin to evaluate isolated protein interactions in vitro.
  • Measured adenosinetriphosphatase (ATPase) kinetic rates in the presence and absence of actin.
  • Actin directly activated the adenosinetriphosphatase (ATPase) activity of isolated myosin subfragment 1.
  • Demonstrated that the subfragment 1 domain alone contains the catalytic and actin-binding sites necessary for actin-activated ATP hydrolysis.

Cite This Study

Eisenberg et al. (1968) studied this question.

synapsesocial.com/papers/6a75204501b447f0dadc036fhttps://doi.org/10.1021/bi00849a022
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

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