November 1, 1994Journal of VirologyOpen Access

Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats

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Population

Adeno-associated virus type 2 inverted terminal repeats and Rep68 protein

Comparison

Truncated forms of ITR and oligonucleotide probes vs Wild-type hairpin ITR

Design

Preclinical

Authors

JCJohn A. ChioriniNational Institutes of Health SWS M WienerBrandeis University ROR. A. OwensNational Institutes of Health

Discussion

Member takes

Implication

Informs AAV vector engineering; hypothesis-generating for clinical gene therapy translation.

Structured PICO

Population

Adeno-associated virus type 2 inverted terminal repeats (ITRs) and Rep68 protein (recombinant fusion MBP-Rep68 delta produced in E. coli and wild-type Rep68)

Intervention

Truncated forms of ITR (delta 57 ITR, delta 28 ITR) and oligonucleotide probes

Comparator

Wild-type hairpin ITR

Outcome

Binding affinity and trs endonuclease activity (nicking and covalent linkage)surrogate

High-affinity binding of Rep68 to AAV2 ITRs requires stabilization by flanking sequences and the intact GCTC repeat motif, while the hairpin portion is necessary for efficient trs cleavage activity.

Cite This Study

Chiorini et al. (1994) studied this question.

synapsesocial.com/papers/6a754d34756843cacbb1f3e1 https://doi.org/10.1128/jvi.68.11.7448-7457.1994

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Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider